Confocal Microscope: Zeiss LSM 5 Pascal AxioImager.M1 (upright configuration)
Location: Broad CIRM Center (BCC) 205A (Pascal)
Description: The LSM 5 Pascal are designed for routine confocal work and are very similar.
The Pascal, located in room 205A, has a 40x long working distance water dipping lens and temperature controlled stage and lens collar, which can be used for maintaining physiological temperatures.
The microscopes also have 2 fluorescent detectors + 1 transmitted detector.
The LSM 5 Pascal are controlled by ZEN 2009 software.
|LSM 5 Pascal Objectives||LSM 5 Exciter Objectives||Laser Lines|
|10x 0.45 NA||10x 0.45 NA||405 nm|
|20x 0.8 NA||20x 0.8 NA||458 nm|
|40x 0.8 NA (water dipping)||488 nm|
|40x 1.3 NA (oil immersion)||40x 1.3 NA (oil immersion)||514 nm|
|63x 1.4 NA (oil immersion)||63x 1.4 NA (oil immersion)||543 nm|