University of Southern California

Microscopy Core Facility

USC Stem Cell

Wide-field Fluorescent Microscopes: Zeiss AxioObserver.A1 and Axiovert 200 (inverted configuration)

Locations: Broad CIRM Center (BCC) 205A, 303C, 409C and 509C

Description: These microscopes are typically used for assessing TC plates and flasks.

The AxioObserver.A1 in 205A is equipped with Narishige hydraulic micromanipulators and a Xyclone IR laser ablation system. This setup can be used for microinjection, microsurgery, dissection and laser ablation.

Camera: AxioCam MRm monochrome camera

Accessories: AxioObserver.A1 in 205A equipped with Narishige micromanipulators and Xyclone IR laser dissection

AxioObserver Objectives (location 205A) AxioObserver Objectives (location 303C) AxioObserver Objectives (location 409C) Axiovert Objectives (location 509C)
2.5x 0.06 NA
5x 0.12 NA phase 0 5x 0.12 NA phase 0 5x 0.12 NA phase 0 4x 0.16 NA
10x 0.3 NA phase 1 10x 0.3 NA phase 1 10x 0.3 NA phase 1 10x 0.3 NA phase 1
20x 0.4 NA phase 2 20x 0.4 NA phase 2 20x 0.4 NA phase 2 20x 0.4 NA phase 2
40x 0.6 NA LWD laser objective 40x 0.6 NA phase 2 40x 0.6 NA phase 2
Axiovert crop