Description: Leica’s Thunder imaging systems remove out-of-focus haze typical of widefield images. Computational Clearing produces sharp, high-contrast images significantly faster than confocal imaging. This instrument is well-suited for specimens that do not require optical sectioning, but would benefit from “haze” reduction. The system captures four color fluorescence, brightfield and DIC. Illumination and detection filters are independently controlled. Stage-top incubation is available for live imaging.

Objectives	Illumination	Detection
5x 0.12 NA	395 nm	Blue       (e.g., Dapi)
10x 0.45 NA	440 nm
20x 0.80 NA	470 nm	Green    (e.g., Alexa 488/GFP)
63X 1.4 NA (oil immersion)	510 nm
	550 nm	Red        (e.g., Alexa 555/RFP)
	640 nm	Far Red (e.g., Alex 647)